Challenges in 3D Live Cell Imaging
نویسندگان
چکیده
A short overview on 3D live cell imaging is given. Relevant samples are described and various problems challenges—including by optical sectioning, light scattering phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples applications summarized. In the future one may profit from a continuous increase in microscopic resolution, but also molecular sensing techniques nanometer range using e.g., non-radiative energy transfer (FRET).
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ژورنال
عنوان ژورنال: Photonics
سال: 2021
ISSN: ['2304-6732']
DOI: https://doi.org/10.3390/photonics8070275